Site-specific transgene integration in chimeric antigen receptor (CAR) T cell therapies.

ABSTRACT: Chimeric antigen receptor (CAR) T cells and natural killer (NK) cells are genetically engineered immune cells that can detect target antigens on the surface of target cells and eliminate them following adoptive transfer. Recent progress in CAR-based therapies has led to outstanding clinical success in certain patients with leukemias and lymphomas and offered therapeutic benefits to those resistant to conventional therapies. The universal approach to stable CAR transgene delivery into the T/NK cells is the use of viral particles. Such approaches mediate semi-random transgene insertions spanning the entire genome with a high preference for integration into sites surrounding highly-expressed genes and active loci. Regardless of the variable CAR expression level based on the integration site o

SUBMITTER: Dabiri H 

PROVIDER: S-EPMC10318782 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature