Oxidative stress drives mutagenesis through transcription-coupled repair in bacteria.

ABSTRACT: In bacteria, mutations lead to the evolution of antibiotic resistance, which is one of the main public health problems of the twenty-first century. Therefore, determining which cellular processes most frequently contribute to mutagenesis, especially in cells that have not been exposed to exogenous DNA damage, is critical. Here, we show that endogenous oxidative stress is a key driver of mutagenesis and the subsequent development of antibiotic resistance. This is the case for all classes of antibiotics and highly divergent species tested, including patient-derived strains. We show that the transcription-coupled repair pathway, which uses the nucleotide excision repair proteins (TC-NER), is responsible for endogenous oxidative stress-dependent mutagenesis and subsequent evolution. This sugge

SUBMITTER: Carvajal-Garcia J 

PROVIDER: S-EPMC10318952 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature