Oviductal extracellular vesicles from women with endometriosis impair embryo development

ABSTRACT:

Objective

To investigate the influence of oviductal extracellular vesicles from patients with endometriosis on early embryo development.

Design

In vitro experimental study

Setting

University-affiliated hospital.

Patients

Women with and without endometriosis who underwent hysterectomy (n = 27 in total).

Interventions

None.

Main outcome measures

Oviductal extracellular vesicles from patients with endometriosis (oEV-EMT) or without endometriosis (oEV-ctrl) were isolated and co-cultured with two-cell murine embryos for 75 hours. Blastocyst rates were recorded. RNA sequencing was used to identify the differentially expressed genes in blastocysts cultured either with oEV-EMT or with oEV-ctrl. Kyoto Encyclopedia of Genes and Genomes (KEGG) pat

SUBMITTER: Li Y 

PROVIDER: S-EPMC10319124 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature