Changes in Medicago truncatula seed proteome along the rehydration-dehydration cycle highlight new players in the genotoxic stress response.

ABSTRACT:

Introduction

Several molecular aspects underlying the seed response to priming and the resulting vigor profile are still poorly understood. Mechanisms involved in genome maintenance deserve attention since the balance between stimulation of germination and DNA damage accumulation versus active repair is a key determinant for designing successful seed priming protocols.

Methods

Changes in the Medicago truncatula seed proteome were investigated in this study, using discovery mass spectrometry and label-free quantification, along the rehydration-dehydration cycle of a standard vigorization treatment (hydropriming plus dry-back), and during post-priming imbibition.

Resuts and discussion

From 2056 to 2190 proteins were detected in each pairwise comparison, among which six

SUBMITTER: Pagano A 

PROVIDER: S-EPMC10319343 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature