Evaluation of Non-invasive Optogenetic Stimulation with Transcranial Functional Ultrasound Imaging.

ABSTRACT: Optogenetics employs engineered viruses to genetically modify cells to express specific light-sensitive ion channels. The standard method for gene delivery in the brain involves invasive craniotomies that expose the brain and direct injections of viruses that invariably damage neural tissue along the syringe tract. A recently proposed alternative in which non-invasive optogenetics is performed with focused ultrasound (FUS)-mediated blood-brain barrier (BBB) openings has been found to non-invasively facilitate gene delivery for optogenetics in mice. Although gene delivery can be performed non-invasively, validating successful viral transduction and expression of encoded ion channels in target tissue typically involves similar invasive techniques, such as craniotomies in longitudinal studies

SUBMITTER: Aurup C 

PROVIDER: S-EPMC10319350 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature