Specific and label-free endogenous signature of dystrophic muscle by Synchrotron deep ultraviolet radiation.

ABSTRACT: Dystrophic muscle is characterized by necrosis/regeneration cycles, inflammation, and fibro-adipogenic development. Conventional histological stainings provide essential topographical data of this remodeling but may be limited to discriminate closely related pathophysiological contexts. They fail to mention microarchitecture changes linked to the nature and spatial distribution of tissue compartment components. We investigated whether label-free tissue autofluorescence revealed by Synchrotron deep ultraviolet (DUV) radiation could serve as an additional tool for monitoring dystrophic muscle remodeling. Using widefield microscopy with specific emission fluorescence filters and microspectroscopy defined by high spectral resolution, we analyzed samples from healthy dogs and two groups of dyst

SUBMITTER: Dubreil L 

PROVIDER: S-EPMC10319894 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature