An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in Caenorhabditis elegans.

ABSTRACT: The auxin-inducible degradation system has been widely adopted in the Caenorhabditis elegans research community for its ability to empirically control the spatiotemporal expression of target proteins. This system can efficiently degrade auxin-inducible degron (AID)-tagged proteins via the expression of a ligand-activatable AtTIR1 protein derived from A. thaliana that adapts target proteins to the endogenous C. elegans proteasome. While broad expression of AtTIR1 using strong, ubiquitous promoters can lead to rapid degradation of AID-tagged proteins, cell type-specific expression of AtTIR1 using spatially restricted promoters often results in less efficient target protein degradation. To circumvent this limitation, we have developed an FLP/FRT3-based system that functions to reanimate a dor

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC10319979 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature