Digital microfluidics-engaged automated enzymatic degradation and synthesis of oligosaccharides.

ABSTRACT: Glycans are an important group of natural biopolymers, which not only play the role of a major biological energy resource but also as signaling molecules. As a result, structural characterization or sequencing of glycans, as well as targeted synthesis of glycans, is of great interest for understanding their structure-function relationship. However, this generally involves tedious manual operations and high reagent consumptions, which are the main technical bottlenecks retarding the advances of both automatic glycan sequencing and synthesis. Until now, automated enzymatic glycan sequencers or synthesizers are still not available on the market. In this study, to promote the development of automation in glycan sequencing or synthesis, first, programmed degradation and synthesis of glycans cat

SUBMITTER: Sun Y 

PROVIDER: S-EPMC10320006 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature