DBALM: A novel method for identifying ornamental flowering plants based on DNA barcodes-leaf morphology.

ABSTRACT: Whereas the presence of flowers on ornamental flowering plants is essential for their identification via traditional methods, ornamental flowering plants cannot be reliably identified in non-flowering stages likewise. Here, DBALM (DNA Barcodes-Leaf Morphology), a new approach that combines DNA barcoding data with micromorphological features of the leaf epidermis and that is not limited by the flowering stage, was used to identify 16 evergreen rhododendron cultivars. First, the sequences of DNA barcodes, ITS, matK, psbA-trnH, and rbcL, were obtained from the DNA of leaves. Phylogenetic analysis was conducted to clarify the groupings among all the samples based on the four markers. Then, microscopic features of the leaf epidermis were used to

SUBMITTER: Shen F 

PROVIDER: S-EPMC10320046 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature