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GenomeFLTR: filtering reads made easy.


ABSTRACT: In the last decade, advances in sequencing technology have led to an exponential increase in genomic data. These new data have dramatically changed our understanding of the evolution and function of genes and genomes. Despite improvements in sequencing technologies, identifying contaminated reads remains a complex task for many research groups. Here, we introduce GenomeFLTR, a new web server to filter contaminated reads. Reads are compared against existing sequence databases from various representative organisms to detect potential contaminants. The main features implemented in GenomeFLTR are: (i) automated updating of the relevant databases; (ii) fast comparison of each read against the database; (iii) the ability to create user-specified databases; (iv) a user-friendly interactive dashboard to investigate the origin and frequency of the contaminations; (v) the generation of a contamination-free file. Availability: https://genomefltr.tau.ac.il/.

SUBMITTER: Dotan E 

PROVIDER: S-EPMC10320065 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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GenomeFLTR: filtering reads made easy.

Dotan Edo E   Alburquerque Michael M   Wygoda Elya E   Huchon Dorothée D   Pupko Tal T  

Nucleic acids research 20230701 W1


In the last decade, advances in sequencing technology have led to an exponential increase in genomic data. These new data have dramatically changed our understanding of the evolution and function of genes and genomes. Despite improvements in sequencing technologies, identifying contaminated reads remains a complex task for many research groups. Here, we introduce GenomeFLTR, a new web server to filter contaminated reads. Reads are compared against existing sequence databases from various represe  ...[more]

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