A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron cultures in vitro.

ABSTRACT:

Introduction

Glass coverslips are used as a substrate since Harrison's initial nerve cell culture experiments in 1910. In 1974, the first study of brain cells seeded onto polylysine (PL) coated substrate was published. Usually, neurons adhere quickly to PL coating. However, maintaining cortical neurons in culture on PL coating for a prolonged time is challenging.

Methods

A collaborative study between chemical engineers and neurobiologists was conducted to find a simple method to enhance neuronal maturation on poly-D-lysine (PDL). In this work, a simple protocol to coat PDL efficiently on coverslips is presented, characterized, and compared to a conventional adsorption method. We studied the adhesion and maturation of primary cortical neurons with various morphological and fu

SUBMITTER: Stil A 

PROVIDER: S-EPMC10320290 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature