Comprehensive immune landscape of lung-resident memory CD8⁺ T cells after influenza infection and reinfection in a mouse model.

ABSTRACT:

Background

Resident phenotypic memory CD8⁺ T cells are crucial for immune defense against pathogens. However, little is known about the potential transitions and regulation mechanisms of their function after influenza virus infection and reinfection. In this study, we utilized integrated transcriptome data and in vivo experiments to investigate the key characteristics behind it.

Methods

Two single-cell RNA sequencing (scRNA-seq) datasets of lung CD8⁺ T cells and one RNA-seq dataset of lung tissue after infection or reinfection were included. After Seurat procedures classifying CD8⁺ T subsets, the scCODE algorithm was used to identify the differentially expressed genes for GSVA, GO, and KEGG pathway enrichment. Monocle 3 and CellChat we

SUBMITTER: Jia J 

PROVIDER: S-EPMC10320391 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature