Genetic Pathways in Peritoneal Mesothelioma Tumorigenesis.

ABSTRACT:

Background/aim

Mesotheliomas are tumors similar to, and probably derived from, mesothelial cells. They carry acquired chromosomal rearrangements, deletions affecting CDKN2A, pathogenetic polymorphisms in NF2, and fusion genes which often contain the promiscuous EWSR1, FUS, and ALK as partner genes. Here, we report the cytogenomic results on two peritoneal mesotheliomas.

Materials and methods

Both tumors were examined using G-banding with karyotyping and array comparative genomic hybridization (aCGH). One of them was further investigated with RNA sequencing, reverse transcription polymerase chain reaction (RT-PCR), Sanger sequencing, and fluorescence in situ hybridization (FISH).

Results

In the first mesothelioma, the karyotype was 25∼26,X,+5,+7,+20[cp4]/50∼52,idemx2[c

SUBMITTER: Panagopoulos I 

PROVIDER: S-EPMC10320557 | biostudies-literature | 2023 Jul-Aug

REPOSITORIES: biostudies-literature