Comparison of three DNA extraction methods for recovery of microbial DNA from Arctic permafrost.
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ABSTRACT:
SUBMITTER: Feng S
PROVIDER: S-EPMC10320573 | biostudies-literature | 2023
REPOSITORIES: biostudies-literature
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Comparison of three DNA extraction methods for recovery of microbial DNA from Arctic permafrost.
Feng Sarah S DeKlotz Marla M Taş Neslihan N
microPublication biology 20230621
Permafrost soils, which contain one of Earth's largest terrestrial carbon stocks, are vulnerable to thaw and microbial decomposition, exacerbating climate change. Advancements in sequencing technologies have facilitated the identification and functional profiling of microbial communities in permafrost, but DNA extraction from these soils is challenging due to their high microbial diversity and low biomass. This study assessed the effectiveness of the DNeasy PowerSoil Pro kit in extracting DNA fr ...[more]
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