Characterization of tumor-associated reactive astrocytes in gliomas by single-cell and bulk tumor sequencing.

ABSTRACT:

Objective

Astrocytes constitute approximately 30% of cells in gliomas and play important roles in synapse construction and survival. Recently, JAK/STAT pathway activation associated with a new type of astrocyte was reported. However, the implications of these tumor-associated reactive astrocytes (TARAs) in glioma are not known.

Methods

We comprehensively assessed TARAs in gliomas, both in single cells and at the bulk tumor level, by analyzing five independent datasets. First, we analyzed two single-cell RNA sequencing datasets of 35,563 cells from 23 patients to estimate the infiltration level of TARAs in gliomas. Second, we collected clinical information and genomic and transcriptomic data of 1,379 diffuse astrocytoma and glioblastoma samples from the Chinese Glioma Genome

SUBMITTER: Zhang CB 

PROVIDER: S-EPMC10320578 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature