High-throughput longitudinal electrophysiology screening of mature chamber-specific hiPSC-CMs using optical mapping.

ABSTRACT: hiPSC-CMs are being considered by the Food and Drug Administration and other regulatory agencies for in vitro cardiotoxicity screening to provide human-relevant safety data. Widespread adoption of hiPSC-CMs in regulatory and academic science is limited by the immature, fetal-like phenotype of the cells. Here, to advance the maturation state of hiPSC-CMs, we developed and validated a human perinatal stem cell-derived extracellular matrix coating applied to high-throughput cell culture plates. We also present and validate a cardiac optical mapping device designed for high-throughput functional assessment of mature hiPSC-CM action potentials using voltage-sensitive dye and calcium transients using calcium-sensitive dyes or genetically encoded calcium indicators (GECI, GCaMP6). We utili

SUBMITTER: Allan A 

PROVIDER: S-EPMC10320609 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature