FTO-dependent m⁶A regulates muscle fiber remodeling in an NFATC1-YTHDF2 dependent manner.

ABSTRACT:

Background

Adolescent idiopathic scoliosis (AIS) is characterized by low lean mass without vertebral deformity. The cause-and-effect relationship between scoliosis and paraspinal muscle imbalance has long puzzled researchers. Although FTO has been identified as a susceptibility gene for AIS, its potential role in the asymmetry of paraspinal muscles has not been fully elucidated.

Methods

We investigated the role of Fto in murine myoblast proliferation, migration, and myogenic differentiation. We examined its precise regulatory influence on murine muscle fiber remodeling in vitro and in vivo. We identified the downstream target gene of Fto by screening key regulators of murine muscle fiber remodeling and identified its m⁶A reader. Deep paraspinal muscle samples were

SUBMITTER: Wang W 

PROVIDER: S-EPMC10320966 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature