Regulatory roles of lncRNA PANDAR in breast cancer cell proliferation.

ABSTRACT:

Background

Breast cancer represents the second most deadly malignancy in women, and long noncoding RNAs (lncRNAs) have crucial functions in its development.

Objective

To investigate effects of the promoter of CDKN1A antisense DNA damage-activated RNA (PANDAR) on epithelial-mesenchymal transition (EMT) in breast cancer cells and their proliferation.

Methods

lncRNAs potentially regulating the transcriptional activity of the E-cadherin (E-cad, an epithelial cell marker) gene promoter were screened using a dual-luciferase reporter assay. PANDAR was overexpressed in Michigan cancer foundation 7 (MCF-7) breast cancer cells. E-cad and N-cadherin (N-cad, a mesenchymal cell marker) levels were detected by immunoblotting. Cell viability was assessed using a

SUBMITTER: Sun Q 

PROVIDER: S-EPMC10321221 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature