Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine.

ABSTRACT:

Introduction

Serious infections of Pseudomonas aeruginosa (PA) in hospitals and the emergence and increase of multidrug resistance have raised an urgent need for effective vaccines. However, no vaccine has been approved to date. One possible reason for this is the limited immune response due to the lack of an efficient delivery system. Self-assembled ferritin nanoparticles are good carriers of heterogeneous antigens, which enhance the activation of immunological responses.

Methods

In this study, two well-studied antigen candidates, PcrV and OprI, were selected and connected to the ferritin nanoparticle by the Spytag/SpyCatcher system to generate the nanovaccine rePO-FN.

Results

Compared to recombinant PcrV-OprI formulated with aluminum adjuvants, intramuscular

SUBMITTER: Li Y 

PROVIDER: S-EPMC10321299 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature