A robotic system for automated genetic manipulation and analysis of Caenorhabditis elegans.

ABSTRACT: The nematode Caenorhabditis elegans is one of the most widely studied organisms in biology due to its small size, rapid life cycle, and manipulable genetics. Research with C. elegans depends on labor-intensive and time-consuming manual procedures, imposing a major bottleneck for many studies, especially for those involving large numbers of animals. Here, we describe a general-purpose tool, WormPicker, a robotic system capable of performing complex genetic manipulations and other tasks by imaging, phenotyping, and transferring C. elegans on standard agar media. Our system uses a motorized stage to move an imaging system and a robotic arm over an array of agar plates. Machine vision tools identify animals and assay developmental stage, morphology, sex, expression of fluo

SUBMITTER: Li Z 

PROVIDER: S-EPMC10321491 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature