Fingerprinting eukaryotic metabolism across the animal kingdom using position-specific isotope analysis (PSIA) ¹³C/¹²C measurements.

ABSTRACT: Despite differences in their overall metabolism, eukaryotes share a common mitochondrial biochemistry. We investigated how this fundamental biochemistry supports overall metabolism using a high-resolution carbon isotope approach, position-specific isotope analysis. We measured carbon isotope ¹³C/¹²C cycling in animals, focusing on amino acids that are formed in mitochondrial reactions and are most metabolically active. Carboxyl isotope determinations for amino acids showed strong signals related to common biochemical pathways. Contrasting isotope patterns were measured for metabolism associated with major life history patterns, including growth and reproduction. Turnover of proteins and lipids as well as gluoconeogensis dynamics could be estimated for these metabolic

SUBMITTER: Fry B 

PROVIDER: S-EPMC10321734 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature