Multi-generation study of heavy ion beam-induced mutations and agronomic trait variations to accelerate rice breeding.

ABSTRACT: Heavy ion beam (HIB) is an effective physical mutagen that has been widely used in plant mutational breeding. Systemic knowledge of the effects caused by different HIB doses at developmental and genomic levels will facilitate efficient breeding for crops. Here we examined the effects of HIB systematically. Kitaake rice seeds were irradiated by ten doses of carbon ion beams (CIB, 25 - 300 Gy), which is the most widely used HIB. We initially examined the growth, development and photosynthetic parameters of the M₁ population and found that doses exceeding 125 Gy caused significant physiological damages to rice. Subsequently, we analyzed the genomic variations in 179 M₂ individuals from six treatments (25 - 150 Gy) via whole-genome sequencing (WGS). The mutation ra

SUBMITTER: Ren W 

PROVIDER: S-EPMC10322207 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature