Cyclic voltammetry, square wave voltammetry or electrochemical impedance spectroscopy? Interrogating electrochemical approaches for the determination of electron transfer rates of immobilized redox proteins.

ABSTRACT: In this article, we cross-examine three well-established electrochemical approaches, namely cyclic voltammetry (CV), cyclic square-wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS) to dissect the electron transfer (ET) rate of electrostatically immobilized cytochrome c on Ag electrodes. A detailed analysis supported by simulations of redox transition provided three distinct values for the heterogeneous electron transfer (HET) rate constant of cyt c interfaced on COOH-terminated C₁₀-long alkanethiol, i.e., k_HET= 47.8 (±2,91) s^-1 in CV, k_HET= 64.8 (±1,27) s^-1 in SWV, and k_HET= 26.5 s^-1 in EIS. We discuss the obtained discrepancies obtained from electrochemical methods and c

SUBMITTER: Pilz FH 

PROVIDER: S-EPMC10322675 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature