Assimilatory sulfate reduction in the marine methanogen Methanothermococcus thermolithotrophicus.

ABSTRACT: Methanothermococcus thermolithotrophicus is the only known methanogen that grows on sulfate as its sole sulfur source, uniquely uniting methanogenesis and sulfate reduction. Here we use physiological, biochemical and structural analyses to provide a snapshot of the complete sulfate reduction pathway of this methanogenic archaeon. We find that later steps in this pathway are catalysed by atypical enzymes. PAPS (3'-phosphoadenosine 5'-phosphosulfate) released by APS kinase is converted into sulfite and 3'-phosphoadenosine 5'-phosphate (PAP) by a PAPS reductase that is similar to the APS reductases of dissimilatory sulfate reduction. A non-canonical PAP phosphatase then hydrolyses PAP. Finally, the F₄₂₀-dependent sulfite reductase converts sulfite to sulfide for cellular assimilati

SUBMITTER: Jespersen M 

PROVIDER: S-EPMC10322725 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature