Successful editing and maintenance of lactogenic gene expression in primary bovine mammary epithelial cells.

ABSTRACT: In vitro investigation of bovine lactation processes is limited by a lack of physiologically representative cell models. This deficiency is most evident through the minimal or absent expression of lactation-specific genes in cultured bovine mammary tissues. Primary bovine mammary epithelial cells (pbMECs) extracted from lactating mammary tissue and grown in culture initially express milk protein transcripts at relatively representative levels. However, expression drops dramatically after only three or four passages, which greatly reduces the utility of primary cells to model and further examine lactogenesis. To investigate the effects of alternate alleles in pbMECs including effects on transcription, we have developed methods to deliver CRISPR-Cas9 gene editing reagents to primary mammary

SUBMITTER: Moody J 

PROVIDER: S-EPMC10322751 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature