Optimal slice thickness for improved accuracy of quantitative analysis of fluorescent cell and microsphere distribution in cryo-images.

ABSTRACT: Cryo-imaging has been effectively used to study the biodistribution of fluorescent cells or microspheres in animal models. Sequential slice-by-slice fluorescent imaging enables detection of fluorescent cells or microspheres for corresponding quantification of their distribution in tissue. However, if slices are too thin, there will be data overload and excessive scan times. If slices are too thick, then cells can be missed. In this study, we developed a model for detection of fluorescent cells or microspheres to aid optimal slice thickness determination. Key factors include: section thickness (X), fluorescent cell intensity (I_fluo), effective tissue attenuation coefficient (μ_T), and a detection threshold (T). The model suggests an optimal slice thickness value that pr

SUBMITTER: Wuttisarnwattana P 

PROVIDER: S-EPMC10322852 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature