Protocol for Bulk-ATAC sequencing in head and neck squamous cell carcinoma.
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ABSTRACT:
SUBMITTER: Chen N
PROVIDER: S-EPMC10323015 | biostudies-literature | 2023 Apr
REPOSITORIES: biostudies-literature
Publications
Protocol for Bulk-ATAC sequencing in head and neck squamous cell carcinoma.
Chen Nana N Vohra Manik M Saladi Srinivas Vinod SV
STAR protocols 20230417 2
The transposase-accessible chromatin using sequencing (ATAC-seq) offers a simplified approach to detect chromatin changes in cancer cells after genetic intervention and drug treatment. Here, we present an optimized ATAC-seq protocol to elucidate chromatin accessibility changes at the epigenetic level in head and neck squamous cell carcinoma cells. We describe steps for cell lysate preparation, transposition, and tagmentation, followed by library amplification and purification. We then detail nex ...[more]
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