An ensemble of cadherin-catenin-vinculin complex employs vinculin as the major F-actin binding mode.

ABSTRACT: The cell-cell adhesion cadherin-catenin complexes recruit vinculin to the adherens junction (AJ) to modulate the mechanical couplings between neighboring cells. However, it is unclear how vinculin influences the AJ structure and function. Here, we identified two patches of salt bridges that lock vinculin in the head-tail autoinhibited conformation and reconstituted the full-length vinculin activation mimetics bound to the cadherin-catenin complex. The cadherin-catenin-vinculin complex contains multiple disordered linkers and is highly dynamic, which poses a challenge for structural studies. We determined the ensemble conformation of this complex using small-angle x-ray and selective deuteration/contrast variation small-angle neutron scattering. In the complex, both α-catenin and vinculin a

SUBMITTER: Shi B 

PROVIDER: S-EPMC10323030 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature