Current state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems.

ABSTRACT: Heterologous expression of L-asparaginase (L-ASNase) has become an important area of research due to its clinical and food industry applications. This review provides a comprehensive overview of the molecular and metabolic strategies that can be used to optimize the expression of L-ASNase in heterologous systems. This article describes various approaches that have been employed to increase enzyme production, including the use of molecular tools, strain engineering, and in silico optimization. The review article highlights the critical role that rational design plays in achieving successful heterologous expression and underscores the challenges of large-scale production of L-ASNase, such as inadequate protein folding and the metabolic burden on host cells. Improved gene expression is

SUBMITTER: Lefin N 

PROVIDER: S-EPMC10323146 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature