DeXtrusion: automatic recognition of epithelial cell extrusion through machine learning in vivo.

ABSTRACT: Accurately counting and localising cellular events from movies is an important bottleneck of high-content tissue/embryo live imaging. Here, we propose a new methodology based on deep learning that allows automatic detection of cellular events and their precise xyt localisation on live fluorescent imaging movies without segmentation. We focused on the detection of cell extrusion, the expulsion of dying cells from the epithelial layer, and devised DeXtrusion: a pipeline based on recurrent neural networks for automatic detection of cell extrusion/cell death events in large movies of epithelia marked with cell contour. The pipeline, initially trained on movies of the Drosophila pupal notum marked with fluorescent E-cadherin, is easily trainable, provides fast and accurate extrusion predictions

SUBMITTER: Villars A 

PROVIDER: S-EPMC10323232 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature