Deep Eutectic Solvent Stir Bar Sorptive Extraction: A Rapid Microextraction Technique for the Determination of Vitamin D₃ by Spectrophotometry.

ABSTRACT: In this paper, a new microextraction method, named deep eutectic solvent stir bar sorptive extraction (DES-SBSE), is described that utilizes a hydrophobic DES (hDES) as a coating for SBSE. As a model, vitamin D₃ was extracted efficiently by this technique from different real samples before its spectrophotometric determination. A conventional magnet was encapsulated inside a glass bar (1.0 cm × 2 mm) and coated by the hDES consisting of tetrabutylammonium chloride and heptadecanoic acid (mole ratio 1:2). Parameters affecting microextraction were studied and optimized by one-variable-at-a-time, central composite design, and Box-Behnken design methods. Under the optimum conditions, the detection limit of 0.08 μg L^-1 could be reached. The linear range of the method was be

SUBMITTER: Hashemi SH 

PROVIDER: S-EPMC10324053 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature