Compound-Specific 1D ¹H NMR Pulse Sequence Selection for Metabolomics Analyses.

ABSTRACT: NMR-based metabolomics approaches have been used in a wide range of applications, for example, with medical, plant, and marine samples. One-dimensional (1D) ¹H NMR is routinely used to find out biomarkers in biofluids such as urine, blood plasma, and serum. To mimic biological conditions, most NMR studies have been carried out in an aqueous solution where the high intensity of the water peak is a major problem in obtaining a meaningful spectrum. Different methods have been used to suppress the water signal, including 1D Carr-Purcell-Meiboom-Gill (CPMG) presat, consisting of a T₂ filter to suppress macromolecule signals and reduce the humped curve in the spectrum. 1D nuclear Overhauser enhancement spectroscopy (NOESY) is another method for water suppression that

SUBMITTER: Singh U 

PROVIDER: S-EPMC10324067 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature