Development of microsatellite markers for the invasive mosquito Aedes koreicus.

ABSTRACT:

Background

Aedes koreicus is a mosquito species native to East Asia which has recently invaded several countries in Europe. In Italy, this mosquito was first detected in the North-East in 2011 and is now widely distributed in the entire northern part of the country. The development of specific genetic markers, such as microsatellites, is necessary to uncover the dispersal routes of this mosquito from its native areas and, eventually, to plan future control interventions.

Methods

Available raw sequences of genomic DNA of Ae. koreicus were screened in silico using BLASTn to identify possible microsatellite-containing sequences. Specific primer pairs were then designed, and their efficiency was determined through polymerase chain reaction (PCR) on 32 individuals of Ae. koreicus

SUBMITTER: Soresinetti L 

PROVIDER: S-EPMC10324130 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature