A rapid and robust leaf ablation method to visualize bundle sheath cells and chloroplasts in C₃ and C₄ grasses.

ABSTRACT:

Background

It has been proposed that engineering the C₄ photosynthetic pathway into C₃ crops could significantly increase yield. This goal requires an increase in the chloroplast compartment of bundle sheath cells in C₃ species. To facilitate large-scale testing of candidate regulators of chloroplast development in the rice bundle sheath, a simple and robust method to phenotype this tissue in C₃ species is required.

Results

We established a leaf ablation method to accelerate phenotyping of rice bundle sheath cells. The bundle sheath cells and chloroplasts were visualized using light and confocal laser microscopy. Bundle sheath cell dimensions, chloroplast area and chloroplast number per cell were measured from the images obtained b

SUBMITTER: Billakurthi K 

PROVIDER: S-EPMC10324140 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature