Dysregulated Rbfox2 produces aberrant splicing of Ca_V1.2 calcium channel in diabetes-induced cardiac hypertrophy.

ABSTRACT:

Background

L-type Ca²⁺ channel Ca_V1.2 is essential for cardiomyocyte excitation, contraction and gene transcription in the heart, and abnormal functions of cardiac Ca_V1.2 channels are presented in diabetic cardiomyopathy. However, the underlying mechanisms are largely unclear. The functions of Ca_V1.2 channels are subtly modulated by splicing factor-mediated alternative splicing (AS), but whether and how Ca_V1.2 channels are alternatively spliced in diabetic heart remains unknown.

Methods

Diabetic rat models were established by using high-fat diet in combination with low dose streptozotocin. Cardiac function and morphology were assessed by echocardiography and HE staining, respectively. Isolated neonatal rat ventricular my

SUBMITTER: Li P 

PROVIDER: S-EPMC10324275 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature