Brincidofovir is a robust replication inhibitor against African swine fever virus in vivo and in vitro.

ABSTRACT: African swine fever virus (ASFV) infection is a major public and socioeconomic concern that has a serious impact on the global swine industry. Unfortunately, there are currently no commercially available vaccines or antiviral agents that are both safe and effective against ASFV. In the study, we use primary porcine alveolar macrophages to screen a kinase inhibitor library for anti-ASFV compounds. Six candidate compounds that inhibited ASFV infection with inhibition of > 90% were identified, among which brincidofovir exhibited optimal inhibitory effects on ASFV. Brincidofovir reduces ASFV replication in a dose-dependent manner (IC₅₀ = 2.76 nM) without cytotoxicity (CC₅₀ = 58 μM). It possesses the ability to reduce viral titres and inhibit viral structural protein expre

SUBMITTER: Guo S 

PROVIDER: S-EPMC10324455 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature