Dynamical scattering in ice-embedded proteins in conventional and scanning transmission electron microscopy.

ABSTRACT: Structure determination of biological macromolecules using cryogenic electron microscopy is based on applying the phase object (PO) assumption and the weak phase object (WPO) approximation to reconstruct the 3D potential density of the molecule. To enhance the understanding of image formation of protein complexes embedded in glass-like ice in a transmission electron microscope, this study addresses multiple scattering in tobacco mosaic virus (TMV) specimens. This includes the propagation inside the molecule while also accounting for the effect of structural noise. The atoms in biological macromolecules are light but are distributed over several nanometres. Commonly, PO and WPO approximations are used in most simulations and reconstruction models. Therefore, dynamical multislice simulations

SUBMITTER: Leidl ML 

PROVIDER: S-EPMC10324487 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature