Genetic characterization and whole-genome sequencing-based genetic analysis of influenza virus in Jining City during 2021-2022.

ABSTRACT:

Background

The influenza virus poses a significant threat to global public health due to its high mutation rate. Continuous surveillance, development of new vaccines, and public health measures are crucial in managing and mitigating the impact of influenza outbreaks.

Methods

Nasal swabs were collected from individuals with influenza-like symptoms in Jining City during 2021-2022. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect influenza A viruses, followed by isolation using MDCK cells. Additionally, nucleic acid detection was performed to identify influenza A H1N1, seasonal H3N2, B/Victoria, and B/Yamagata strains. Whole-genome sequencing was conducted on 24 influenza virus strains, and subsequent analyses included characterization,

SUBMITTER: Li L 

PROVIDER: S-EPMC10324579 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature