Allergen-induced DNA release by the airway epithelium amplifies type 2 immunity.

ABSTRACT:

Background

Alternaria alternata and house dust mite exposure evokes IL-33 secretion from the airway epithelium, which functions as an alarmin to stimulate type 2 immunity. Extracellular DNA (eDNA) is also an alarmin that intensifies inflammation in cystic fibrosis, chronic obstructive pulmonary disease, and asthma.

Objective

We investigated the mechanisms underlying allergen-evoked DNA mobilization and release from the airway epithelium and determined the role of eDNA in type 2 immunity.

Methods

Human bronchial epithelial (hBE) cells were used to characterize allergen-induced DNA mobilization and extracellular release using comet assays to measure DNA fragmentation, Qubit double-stranded DNA assays to measure DNA release, and DNA sequencing to determine eDNA compositi

SUBMITTER: Srisomboon Y 

PROVIDER: S-EPMC10324884 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature