A versatile genomic transgenesis platform with enhanced λ integrase for human Expi293F cells.

ABSTRACT: Reliable cell-based platforms to test and/or produce biologics in a sustainable manner are important for the biotech industry. Utilizing enhanced λ integrase, a sequence-specific DNA recombinase, we developed a novel transgenesis platform involving a fully characterized single genomic locus as an artificial landing pad for transgene insertion in human Expi293F cells. Importantly, transgene instability and variation in expression were not observed in the absence of selection pressure, thus enabling reliable long-term biotherapeutics testing or production. The artificial landing pad for λ integrase can be targeted with multi-transgene constructs and offers future modularity involving additional genome manipulation tools to generate sequential or nearly seamless insertions. We demonstrated br

SUBMITTER: Siddiqui AA 

PROVIDER: S-EPMC10325659 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature