Integrated single-cell analysis-based classification of vascular mononuclear phagocytes in mouse and human atherosclerosis.

ABSTRACT:

Aims

Accumulation of mononuclear phagocytes [monocytes, macrophages, and dendritic cells (DCs)] in the vessel wall is a hallmark of atherosclerosis. Using integrated single-cell analysis of mouse and human atherosclerosis, we here aimed to refine the nomenclature of mononuclear phagocytes in atherosclerotic vessels and to compare their transcriptomic profiles in mouse and human disease.

Methods and results

We integrated 12 single-cell RNA-sequencing (scRNA-seq) datasets of immune cells isolated from healthy or atherosclerotic mouse aortas, and data from 11 patients (n = 4 coronary vessels, n = 7 carotid endarterectomy specimens) from two studies. Integration of mouse data identified subpopulations with discrete transcriptomic signatures within previously described population

SUBMITTER: Zernecke A 

PROVIDER: S-EPMC10325698 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature