FOXK1 regulates Wnt signalling to promote cardiogenesis.

ABSTRACT:

Aims

Congenital heart disease (CHD) is the most common genetic birth defect, which has considerable morbidity and mortality. We focused on deciphering key regulators that govern cardiac progenitors and cardiogenesis. FOXK1 is a forkhead/winged helix transcription factor known to regulate cell cycle kinetics and is restricted to mesodermal progenitors, somites, and heart. In the present study, we define an essential role for FOXK1 during cardiovascular development.

Methods and results

We used the mouse embryoid body system to differentiate control and Foxk1 KO embryonic stem cells into mesodermal, cardiac progenitor cells and mature cardiac cells. Using flow cytometry, immunohistochemistry, cardiac beating, transcriptional and chromatin immunoprecipitation quantitative polyme

SUBMITTER: Sierra-Pagan JE 

PROVIDER: S-EPMC10325700 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature