Manipulation of IME4 expression, a global regulation strategy for metabolic engineering in Saccharomyces cerevisiae.

ABSTRACT: Metabolic engineering has been widely used for production of natural medicinal molecules. However, engineering high-yield platforms is hindered in large part by limited knowledge of complex regulatory machinery of metabolic network. N⁶-Methyladenosine (m⁶A) modification of RNA plays critical roles in regulation of gene expression. Herein, we identify 1470 putatively m⁶A peaks within 1151 genes from the haploid Saccharomyces cerevisiae strain. Among them, the transcript levels of 94 genes falling into the pathways which are frequently optimized for chemical production, are remarkably altered upon overexpression of IME4 (the yeast m⁶A methyltransferase). In particular, IME4 overexpression elevates the mRNA levels of the methylated gen

SUBMITTER: Zhu J 

PROVIDER: S-EPMC10326246 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature