Revealing viral and cellular dynamics of HIV-1 at the single-cell level during early treatment periods.

ABSTRACT: While combination therapy completely suppresses HIV-1 replication in blood, functional virus persists in CD4⁺ T cell subsets in non-peripheral compartments that are not easily accessible. To fill this gap, we investigated tissue-homing properties of cells that transiently appear in the circulating blood. Through cell separation and in vitro stimulation, the HIV-1 "Gag and Envelope reactivation co-detection assay" (GERDA) enables sensitive detection of Gag+/Env+ protein-expressing cells down to about one cell per million using flow cytometry. By associating GERDA with proviral DNA and polyA-RNA transcripts, we corroborate the presence and functionality of HIV-1 in critical body compartments utilizing t-distributed stochastic neighbor embedding (tSNE) and density-based spat

SUBMITTER: Otte F 

PROVIDER: S-EPMC10326345 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature