Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants.

ABSTRACT: A growing interest in the production and commercialization of A2 cow's milk has been observed in many countries in the last few years due to the beneficial properties for human health attributed to A2 β-casein variant. Methods of varying complexity and different equipment requirements have been proposed for the determination of the β-casein genotype of individual cows. We proposed herein a modification of a previously patented method based on an amplification-created restriction site PCR followed by restriction fragment length polymorphism analysis. This method allows to identify and differentiate A2-like from A1-like β-casein variants, after differential endonuclease cleavage flanking the nucleotide that determines the amino acid at position 67 of β-casein. The advantages of this method a

SUBMITTER: Raschia MA 

PROVIDER: S-EPMC10326427 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature