Directed stepwise tracing of polysynaptic neuronal circuits with replication-deficient pseudorabies virus.

ABSTRACT: Brain functions are accomplished by polysynaptic circuits formed by neurons wired together through multiple orders of synaptic connections. Polysynaptic connectivity has been difficult to examine due to a lack of methods of continuously tracing the pathways in a controlled manner. Here, we demonstrate directed, stepwise retrograde polysynaptic tracing by inducible reconstitution of replication-deficient trans-neuronal pseudorabies virus (PRV^ΔIE) in the brain. Furthermore, PRV^ΔIE replication can be temporally restricted to minimize its neurotoxicity. With this tool, we delineate a wiring diagram between the hippocampus and striatum-two major brain systems for learning, memory, and navigation-that consists of projections from specific hippocampal domains to speci

SUBMITTER: Du W 

PROVIDER: S-EPMC10326449 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature