Radiotherapy combined with immunotherapy could improve the immune infiltration of melanoma in mice and enhance the abscopal effect.

ABSTRACT:

Purpose

To analyze the gene mutation, immune infiltration and tumor growth of primary tumor and distant tumor under different treatment modes.

Materials and methods

Twenty B16 murine melanoma cells were injected subcutaneously into the of both sides of the thigh, simulating a primary tumor and a secondary tumor impacted by the abscopal effect, respectively. They were divided into blank control group, immunotherapy group, radiotherapy group, and radiotherapy combined immunotherapy group. During this period, tumor volume was measured, and RNA sequencing was performed on tumor samples after the test. R software was used to analyze differentially expressed genes, functional enrichment, and immune infiltration.

Results

We found that any treatment mode could cause changes i

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC10326504 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature