MiR-410-3p suppresses primary gastric cancer and exosomes regulate endogenous expression of miR-410-3p.

ABSTRACT: MicroRNAs play significant roles in cancer initiation and progression. Exosomes are important extracellular vesicles for transporting molecules to distant sites. This study aims to investigate the functional roles of miR-410-3p in primary gastric cancer, as well as the roles of exosomes in regulating expression of miR-410-3p. In this study, forty-seven pairs of human gastric cancer tissue samples were collected. Endogenous expression of miR-410-3p in tissue samples and cell lines, and expression of exosomal miR-410-3p in cell culture medium were evaluated by RT-qPCR. Functional assays including cell proliferation assay by MTT, cell migration and invasion assay by transwell, and cell adhesion assay were performed. Targets of miR-410-3p were screened. Cell culture medium of culturing cell li

SUBMITTER: Liu MX 

PROVIDER: S-EPMC10326593 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature