Generation of a monoclonal antibody against duck circovirus capsid protein and its potential application for native viral antigen detection.

ABSTRACT:

Introduction

Duck circovirus (DuCV) infection is currently recognized as an important immunosuppressive disease in commercial duck flocks in China. Specific antibodies against DuCV viral proteins are required to improve diagnostic assays and understand the pathogenesis of DuCV infection.

Methods and results

To generate DuCV-specific monoclonal antibodies (mAbs), a recombinant DuCV capsid protein without the first 36 N-terminal amino acids was produced in Escherichia coli. Using the recombinant protein as an immunogen, a mAb was developed that reacted specifically with the DuCV capsid protein, expressed in E. coli and baculovirus systems. Using homology modeling and recombinant truncated capsid proteins, the antibody-binding epitope was mapped within the region

SUBMITTER: Li J 

PROVIDER: S-EPMC10326623 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature