Protein Nucleation and Crystallization Process with Process Analytical Technologies in a Batch Crystallizer.

ABSTRACT: Protein crystallization has drawn great attention to replacing the traditional downstream processing for protein-based pharmaceuticals due to its advantages in stability, storage, and delivery. Limited understanding of the protein crystallization processes requires essential information based on real-time tracking during the crystallization process. A batch crystallizer of 100 mL fitted with a focused beam reflectance measurement (FBRM) probe and a thermocouple was designed for in situ monitoring of the protein crystallization process, with simutaneously record of off-line concentrations and crystal images. Three stages in the protein batch crystallization process were identified: long-period slow nucleation, rapid crystallization, and slow growth and breakage. The induction time was estim

SUBMITTER: Tian W 

PROVIDER: S-EPMC10326882 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature